Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), Brilliant Violet 786, eBioscience
Mouse CD45.1 단백질을 인식하는 A20 클론 단일클론 항체로, Brilliant Violet 786 형광체로 결합되어 흐름세포분석용으로 최적화됨. 405 nm 레이저 사용 장비에 적합하며, 빛에 민감하므로 암조건 보관 필요. 연구용으로만 사용 가능.
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Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), Brilliant Violet 786, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 1.0 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A20 |
| Conjugate | Brilliant Violet™ 786 |
| Excitation / Emission Max | 406 / 788 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2937217 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711
- eFluor 450, 506
- FITC
- NovaFluor Blue 510, 610-30S, 660-40S
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 645, 702
- Custom conjugation available
Product Specific Information
The A20 monoclonal antibody reacts with mouse CD45 (leukocyte common antigen, LCA) in CD45.1-expressing strains such as SJL/J, DA, STS/A, and RIII. CD45.1 is expressed on all leukocytes in these strains.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse splenocytes; ≤1.0 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
Brilliant Violet™ 786 (BV786) is a tandem dye emitting at 786 nm, for use on cytometers equipped with a violet (405 nm) laser.
Recommendations:
- Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific polymer interactions.
- Protect from light (photo-induced oxidation sensitivity).
- Fixation compatible with IC Fixation Buffer (00-8222-49) or Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C.
- Not compatible with methanol-based fixation.
Excitation: 407 nm
Emission: 786 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
CD45 (LCA) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells (~10% of lymphocyte surface proteins). It regulates Src-family kinases, lymphocyte development, and antigen signaling. Multiple isoforms exist due to alternative splicing, differing in extracellular domains and expression patterns. CD45 also modulates integrin-mediated signaling and apoptosis-related DNA fragmentation. CD45RA is an isoform with restricted expression among lymphoid subtypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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