Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), Brilliant Violet 786, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711
• eFluor 450, 506
• FITC
• NovaFluor Blue 510, 610-30S, 660-40S
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702
• Custom conjugation available

Product Specific Information

The A20 monoclonal antibody reacts with mouse CD45 (leukocyte common antigen, LCA) in CD45.1-expressing strains such as SJL/J, DA, STS/A, and RIII. CD45.1 is expressed on all leukocytes in these strains.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes; ≤1.0 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)

Brilliant Violet™ 786 (BV786) is a tandem dye emitting at 786 nm, for use on cytometers equipped with a violet (405 nm) laser.

Recommendations:

• Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific polymer interactions.
• Protect from light (photo-induced oxidation sensitivity).
• Fixation compatible with IC Fixation Buffer (00-8222-49) or Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C.
• Not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 786 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD45 (LCA) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells (~10% of lymphocyte surface proteins). It regulates Src-family kinases, lymphocyte development, and antigen signaling. Multiple isoforms exist due to alternative splicing, differing in extracellular domains and expression patterns. CD45 also modulates integrin-mediated signaling and apoptosis-related DNA fragmentation. CD45RA is an isoform with restricted expression among lymphoid subtypes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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