Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Brilliant Violet 786, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 563, 661, 737, 805
• Brilliant Violet 421, 650, 711
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue Series (585, 610-70S, 660-120S, 760)
• Pacific Orange
• PE and PE derivatives (Cyanine5, Cyanine5.5, Cyanine7, eFluor 610)
• PerCP derivatives (Cyanine5.5, eFluor 710)
• Super Bright Series (436, 600, 645, 702, 780)
• Custom conjugation available

Product Specific Information

The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage cells and activated mature T and B lymphocytes.
CD25 associates with CD122 (IL-2Rβ) and CD132 (common γ-chain) to form a high-affinity IL-2 receptor. Binding of IL-2 to both high and low affinity classes is inhibited by PC61.5 antibody.
This antibody is validated for flow cytometric analysis of mouse splenocytes and recommended at ≤ 0.5 µg/test (100 µL final volume).

Brilliant Violet™ 786 (BV786) emits at 786 nm and is suitable for cytometers equipped with a 405 nm violet laser.
Use Super Bright Complete Staining Buffer or Brilliant Stain Buffer™ to minimize non-specific polymer interactions when multiple polymer dyes are used.

Light sensitivity: Protect vial and stained samples from light.
Fixation compatibility: Samples can be stored up to 3 days at 4°C using IC Fixation Buffer or 1-step Fix/Lyse Solution. BV786 is not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 786 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD25 (IL2 receptor alpha chain/IL2RA) plays a role in T and B lymphocyte proliferation. The high-affinity IL2 receptor consists of IL2RA, IL2RB, and IL2RG chains. IL2 is produced primarily by mature T cells and functions as a growth, differentiation, and cell death regulator.
Mutations in IL2RA are linked to interleukin 2 receptor alpha deficiency and immune dysregulation.

For Research Use Only. Not for use in diagnostic procedures.

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