Thermo Fisher Scientific ZO-1 Polyclonal Antibody (ZMD.437)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 21 publications 21 publications

Immunohistochemistry (IHC)

-

View 41 publications 41 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

View 7 publications 7 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

2 µg/mL

View 3 publications 3 publications

Immunohistochemistry - Free Floating (IHC (Free))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5-10 µg/mL

View 49 publications 49 publications

Immunoprecipitation (IP)

-

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 10 publications 10 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Chicken, Dog, Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Clone

ZMD.437

Immunogen

Synthetic peptide derived from the N-terminal region of human, dog, mouse, and rat ZO-1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ZO-1,uniProtId:P39447-1,ncbiNodeId:10090,antigenRange:1,antigenLength:1745,antigenImageFileName:40-2300_ZO-1_P39447-1_Rabbit.svg,antigenImageFileNamePDP:40-2300_ZO-1_P39447-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533457

Product Specific Information

This antibody reacts with the ZO-1 protein. Based on sequence homology, reactivity with ZO-2 or ZO-3 is not expected. 40-2300 has been successfully used in immunofluorescence and immunohistochemistry. However, for western blotting we recommend the use of monoclonal anti-ZO-1 antibody (Cat. No. 339100), or other polyclonal ZO-1 antibodies (Cat. Nos. 617300 or 402200).

Reactivity has been confirmed with MDCK-II, Caco-2, Rat-1, and NRK52E cells by immunofluorescence. Reactivity has also been confirmed with frozen mouse liver, blood vessel (in heart), and mossy fiber terminal (in brain) tissues by immunohistochemistry and immunofluorescence, and with formalin-fixed, paraffin-embedded human pancreas, prostate, and small intestine tissues by immunohistochemistry. This antibody (or Cat. No. 187430) is recommended for immunohistochemical detection of ZO-1.

For immunohistochemistry in formalin-fixed, paraffin-embedded tissues, tissue pretreatment with pepsin is required prior to staining. Some lots of this antibody have successfully been used in western blotting using human, dog and rat cell lysates. For immunofluorescence methanol fixation is preferred even though paraformaldehyde fixation will also work.

Target Information

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.