Thermo Fisher Scientific alpha-Cardiac Actin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:10,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 99 and 354 (P68032) of alpha Actin (cardiac muscle) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:alpha-Cardiac Actin,uniProtId:P68032-1,ncbiNodeId:9606,antigenRange:99-354,antigenLength:377,antigenImageFileName:PA5-21396_alpha-Cardiac_Actin_P68032-1_Rabbit.svg,antigenImageFileNamePDP:PA5-21396_alpha-Cardiac_Actin_P68032-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.39 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11152296

Product Specific Information

Recommended positive controls: Jurkat , Raji, K562, THP-1, NIH-3T3, JC, BCL-1, Rat muscle, PC-12, Rat2.

Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (99%), Pig (100%), Chicken (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ACTC1 are part of actins, which are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.