Thermo Fisher Scientific GAD67 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 5 and 216 of GAD67 (Uniprot ID#Q99259) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GAD67,uniProtId:Q99259-1,ncbiNodeId:9606,antigenRange:5-216,antigenLength:594,antigenImageFileName:PA5-21397_GAD67_Q99259-1_Rabbit.svg,antigenImageFileNamePDP:PA5-21397_GAD67_Q99259-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.22 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11153284

Product Specific Information

Recommended positive controls: SK-N-SH, mouse brain, rat brain, human GAD1-transfected 293T.

Predicted reactivity: Mouse (96%), Rat (96%), Dog (99%), Cat (99%), Pig (99%), Chicken (90%), Chimpanzee (100%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.