Thermo Fisher Scientific UNG Polyclonal Antibody
Thermo Fisher Scientific의 UNG 폴리클로날 항체는 인간, 마우스, 랫트 반응성을 가지며 Western blot 및 IHC(P) 실험에 적합합니다. 항원 친화 크로마토그래피로 정제된 고순도 항체로, PBS 버퍼에 0.02% sodium azide가 포함되어 있습니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 14 amino acid peptide from near the carboxy terminus of human UNG1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long term storage store at –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154820 |
Product Specific Information
- Suggested positive control: C2C12 cell lysate
- PA5-20264 can be used with blocking peptide PEP-0379
Target Information
The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase. These forms are generated by alternative splicing and the use of two differentially regulated promoters, PA and PB.
The cDNAs for UNG1 and UNG2 are of similar size (2061 and 2058 bp, respectively), and the corresponding mRNAs are not resolved as two species in gel electrophoresis.
Nuclear UNG2 differs from mitochondrial UNG1 in 44 amino acids of the N-terminal sequence that is not necessary for catalytic activity.
A major role of UNG gene products is to repair mutagenic U:G mispairs caused by cytosine deamination. For example, UNG2 removes misincorporated dUMP residues.
The level and expression pattern of UNG1 and UNG2 differ between cell and tissue types. Additionally, the expression of UNG is cell cycle regulated.
UNG expression and activity are generally higher in proliferating compared to nonproliferating tissues and cells.
UNG1 is a 304 amino acid protein, while UNG2 is a 313 amino acid protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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