Thermo Fisher Scientific LEF1/TCF1 alpha (Transcription Factor) Recombinant Rabbit Monoclonal Antibody (LEF1, 341R)
인간 LEF1 전사인자에 특이적인 재조합 토끼 단클론 항체. IHC(P)에서 1-2 µg/mL로 사용 가능. HEK293 세포에서 발현되며 Protein A/G로 정제됨. 보존제 없는 PBS(pH 7.4) 용액 형태로 제공, -20°C 이하에서 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | LEF1, 341R |
| Immunogen | Recombinant fragment (around aa100–200) of human LEF1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | –20°C or –80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed):
Incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein can bind to a functionally important site in the T-cell receptor-alpha enhancer, conferring maximal enhancer activity. It is involved in the Wnt signaling pathway and may function in hair cell differentiation and follicle morphogenesis. Mutations have been found in somatic sebaceous tumors and are also linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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