Thermo Fisher Scientific LEF1/TCF1 alpha (Transcription Factor) Recombinant Mouse Monoclonal Antibody (rLEF1, 6906)
LEF1/TCF1 alpha 전사인자를 인식하는 마우스 재조합 단클론 항체. 포르말린 고정 조직의 면역조직화학(IHC) 분석에 적합. Protein A/G 정제, PBS(pH 7.4) 용액, 방부제 없음. -20°C 또는 -80°C 보관 권장.
- 카탈로그번호
- 51176-MSM6-P1ABX
- 판매단위
- pk
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rLEF1, 6906 |
| Immunogen | Recombinant fragment (around aa100–200) of human LEF1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The encoded protein binds to a functionally important site in the T-cell receptor-alpha enhancer, conferring maximal enhancer activity. It is involved in the Wnt signaling pathway and may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been associated with sebaceous tumors and other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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