Thermo Fisher Scientific LEF1/TCF1 alpha (Transcription Factor) Recombinant Mouse Monoclonal Antibody (rLEF1, 6906)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature (RT).
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at RT for 20 minutes.

Target Information

This gene encodes a transcription factor belonging to the high mobility group protein-1 family. The encoded protein binds to a functionally important site in the T-cell receptor-alpha enhancer, conferring maximal enhancer activity. It is involved in the Wnt signaling pathway and may function in hair cell differentiation and follicle morphogenesis. Mutations are associated with somatic sebaceous tumors and other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.