Thermo Fisher Scientific SKA3 Polyclonal Antibody
SKA3 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합합니다. Human, Mouse, Rat에 반응하며, Protein A로 정제된 액상 항체입니다. 세포분열 시 염색체 부착 및 방추체 형성 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human SKA3 (amino acids 201–300) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Upon entry into mitosis, the cell’s microtubule (MT) network forms the mitotic spindle, allowing segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin, termed kinetochores (KT), are essential for proper attachment of chromosomes to spindle MTs.
A spindle and kinetochore complex composed of SKA1, SKA2, and SKA3 proteins is required for stable KT-MT interactions and timely anaphase onset. Depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged metaphase-like state. SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting that the SKA complex plays a key role in spindle checkpoint silencing and maintenance of chromosome cohesion during mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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