Thermo Fisher Scientific CD25 Monoclonal Antibody (CD25-4E3), APC, eBioscience

Thermo Fisher Scientific CD25 Monoclonal Antibody (CD25-4E3), APC, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.06 µg)/test
Publications: View 12 publications

Product Specifications

Available Formats

• APC-eFluor 780
• Brilliant Violet 605
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

This CD25-4E3 monoclonal antibody reacts to human CD25, also known as the low-affinity interleukin (IL)-2 receptor alpha. CD25 is expressed on activated T cells (including regulatory T cells), B cells, and macrophages. It associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high-affinity IL-2 receptor. IL-2 receptor signaling is linked to lymphocyte proliferation and survival. CD25 serves as a marker for Hodgkin’s disease and is associated with autoimmune diseases such as Type I diabetes.

This monoclonal antibody is cross-reactive to rhesus monkey.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
Use at 5 µL (0.06 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test (empirically determined).
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine receptor involved in T and B lymphocyte proliferation. The IL2 receptor is a heterotrimeric complex composed of IL2RA, IL2RB, and IL2RG. Homodimeric IL2RA chains form low-affinity receptors, while IL2RB homodimers yield medium-affinity receptors. IL2 expression in mature thymocytes is monoallelic, serving as a unique regulatory mechanism. IL2, primarily produced by mature T cells, acts as a growth, differentiation, and cell death regulator. It promotes B cell proliferation, enhances NK cell activity, and inhibits granulocyte macrophage colony formation. Mutations in this gene are linked to interleukin 2 receptor alpha deficiency and autoimmune disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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