Thermo Fisher Scientific INSR beta Recombinant Rabbit Monoclonal Antibody (SN20-13)
INSR beta 단백질을 인식하는 토끼 단클론 항체로, Western blot, IHC, ICC/IF에 적합합니다. 사람, 마우스, 랫트에 반응하며, 재조합 형식으로 높은 특이성과 재현성을 제공합니다. 단백질 A로 정제된 액상 형태로, 연구용으로 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SN20-13 |
| Immunogen | Synthetic peptide within human Insulin receptor aa 930–970 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2911587 |
Product Specific Information
- Positive Control: MCF-7 cell lysate, 293 cell lysate, HepG2, LO2, RH-35, PANC-1
- Subcellular Location: Cell membrane
Target Information
Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways through activation of phosphorylation cascades. Insulin/IGF-1 binding to the extracellular domain leads to autophosphorylation of downstream target proteins. These receptors differ in sequence in regions conferring specificity for their ligands and in intracellular signaling domains, resulting in distinct functional outcomes. Defects in the insulin receptor (IR) are linked to insulin resistance syndromes, while IGF-1R defects may cause growth retardation. Both signaling cascades play key roles in cancer development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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