Thermo Scientific™

LanthaScreen™ TR-FRET LXR alpha Coactivator Assay Kit, rabbit

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4655:The LanthaScreen™ TR-FRET LXR (liver X자세히 알아보기

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4655:

The LanthaScreen™ TR-FRET LXR (liver X receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR alpha ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an LXR alpha ligand-binding domain (LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.

In the LanthaScreen™ TR-FRET LXR alpha Coactivator Assay, LXR alpha-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the LXR alpha-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

사양

색상Green

콘주게이트Tb (Terbium)

검출 방법Fluorescence

용도(장비)Microplate Reader

수량800 x 20 μL assays

포장384-well Plate

어세이 카테고리Biochemical Assay

약효를 기대할 수 있는 타겟Nuclear Receptors

판독TR-FRET (Activity)

제품라인LanthaScreen™

용도(애플리케이션)Nuclear Receptor Assay, TR-FRET

리간드LXR alpha

제품 유형TR-FRET LXR Alpha Coactivator Assay Kit

No. of Assays800 x 20 μL Assays

Unit SizeEach