Thermo Fisher Scientific H3K27me3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200

View 1 publication 1 publication

ELISA (ELISA)

1:200

-

Peptide Array (Array)

1:2,000

-

Dot blot (DB)

1:20,000

-

Product Specifications

Species Reactivity

Fruit fly, Flatworm, Human, Mouse, Plant, Pig, Rat, Zebrafish

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

rabbit immunized with the region of the histone H3 containing the trimethylated lysine 27 (H3K27me3)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.6 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.05% sodium azide, 0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

RRID

AB_2534388

Target Information

H3K27me3 is the epigenetic mark where histone H3 is trimethylated at lysine residue 27. H3K27me3 is an epigenetic silencing mark and thus associated with downregulation of nearby genes and often found in heterochromatin regions. H3K27me3 is mainly found at inactive gene promoters and generally is found in opposition to the activating mark H3K4me3. H3K27me3 often occupies gene rich regions with close association to development regulators. EZH2, a component of the PRC2 complex, is the histone methyltransferase (or writer) responsible for trimethylating H3K27. UTX and JMJD3 both are histone demethylases (or erasers) for H3K27me3. The regulation of H3K27me3 is crucial for gene regulation and dysregulation of H3K27me3 has been implicated in numerous diseases including cancer. H3K27me3 antibodies are often used for western blot and ChIP applications to study changes in levels and occupancy of H3K27me3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.