Norgen Urine Exosome Purification and RNA Isolation Midi Kit

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요로 유래 엑소좀과 RNA를 동시에 정제하는 올인원 키트로, 초원심분리나 단백질 처리 없이 빠르고 효율적인 정제 가능. 모든 크기의 RNA(마이크로RNA 포함) 분리 가능하며, 다양한 시료 부피에 대응. 고순도 엑소좀 확보로 NGS, qPCR 등 후속 분석에 최적.

카탈로그번호: 58700
브랜드: Norgen
카테고리: RNA Kits
판매단위: pk

#Norgen #R #Reagents #Exosome Reagents

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Norgen · Norgen Urine Exosome Purification and RNA Isolation Midi Kit

Norgen Urine Exosome Purification and RNA Isolation Midi Kit

SKU: 58700
All-in-one system for purification of exosomes and subsequent isolation of RNA from urine samples.

Features and Benefits

Purifies and enriches intact urinary exosomes for functional studies
Isolates all sizes of RNA, including microRNA, without bias
Supports versatile urine input volumes (2–10 mL)
No phenol extraction, Proteinase K treatment, or carrier RNA required
No ultracentrifugation, filtration, or precipitation reagents needed
Compatible with urine from most species
Produces pure exosomes free from RNA-binding proteins
Spin column chromatography using Norgen’s proprietary resin
Purified RNA suitable for downstream applications (e.g., Small RNA Sequencing, qPCR, RT-PCR)

Product Description

Norgen’s Urine Exosome Purification and RNA Isolation Kits provide an all-in-one system for exosome purification and exosomal RNA isolation from urine. The process is based on spin column chromatography utilizing Norgen’s proprietary resin.
These kits isolate all sizes of RNA, including microRNA, without bias and eliminate the need for special instruments or reagents. Elution volume is flexible (50–100 µL). Purified RNA is of high integrity and suitable for qPCR, RT-PCR, Northern blotting, RNase protection, and expression assays.

Kit Variants

Kit Type	Sample Volume Range
Mini	0.25 – 1 mL
Midi	2 – 10 mL
Maxi	11 – 30 mL

Supporting Data

Figure 1. Isolation of RNA from exosomes purified from different urine volumes

A. Avg. Ct Value of Urinary Exosomal miR-30a

Different Urine Volumes (mL)	Avg. Ct Value
0.25	27.82
0.5	26.88
1.0	26.00

B. Relative Amount of Urinary Exosomal miR-30a

Different Urine Volumes (mL)	Relative Amount	% of Linearity
0.25	0.04	100
0.5	0.08	96
1.0	0.15	92

Figure 2. Comparing exosome yields from different commercial purification methods

A. Concentration (×10⁹ / mL Urine)

Sample	Concentration
Competitor S	10.20
Competitor L	3.57
Norgen	16.72

B. Particle Size Distribution (nm)
X-axis: Particle Size (nm)
Y-axis: Concentration (×10⁷ / mL)
Legend: Competitor L, Competitor S, Norgen
Peak sizes observed: 65, 105, 115, 145 nm

Figure 3. Comparing exosome profiles from Norgen’s method and ultracentrifugation

Method	y-axis	x-axis	Major Particle Sizes (nm)
Ultracentrifugation	Concentration (×10⁸ / mL)	Particle Size	45, 95, 125, 165, 235, 295
Norgen	Concentration (×10⁸ / mL)	Particle Size	45, 95, 145, 195, 245, 295

Figure 5. Isolation of RNA from exosomes purified from different urine volumes (Maxi Kit)

A. Avg Ct. Value of Urinary Exosomal miR-30a

Different Urine Volumes (mL)	Avg Ct. Value
11	21.84
30	20.37

B. Relative Amount of Urinary Exosomal miR-30a

Different Urine Volumes (mL)	Relative Amount	% of Linearity
11	2.67	100
30	7.41	93

Kit Specifications

Specification	Description
Minimum Urine Input	2 mL
Maximum Urine Input	10 mL
Size of Exosomes Purified	40–150 nm
Size of RNA Purified	All sizes, including miRNA and small RNA (<200 nt)
Elution Volume	50–100 μL
Time to Complete 10 Purifications	35–40 minutes
Average Yields*	Variable depending on specimen

*Refer to product insert for yield and quantification details.

Storage Conditions and Stability

Store all buffers tightly sealed at room temperature
Stable for 2 years after shipment
Warm Lysis Buffer A at 60°C for 20 minutes if salt precipitation occurs

Important Note

Urine stored at -70°C, -20°C, or 4°C may form precipitates affecting exosome recovery.
Use Norgen’s Urine Preservative for optimal nucleic acid and protein preservation in fresh urine at room temperature (stable for >2 years).

Kit Components

Component	Cat. 58400 (Mini)	Cat. 58700 (Midi)	Cat. 58800 (Maxi)
Slurry E	12.5 mL	12.5 mL	12.5 mL
ExoC Buffer	8 mL	30 mL	50 mL
ExoR Buffer	12 mL	12 mL	12 mL
Lysis Buffer A	20 mL	20 mL	20 mL
Lysis Additive B	2 mL	2 mL	2 mL
Wash Solution A	18 mL	18 mL	18 mL
Elution Solution A	6 mL	6 mL	6 mL
Mini Filter Spin Columns	50	25	15
Mini Spin Columns	50	25	15
Collection Tubes	50	25	15
Elution Tubes (1.7 mL)	100	50	30
Product Insert	1	1	1

Documentation

Citations

Title	Journal	Authors
Placental exosomes isolated from urine of patients with gestational diabetes exhibit a differential profile expression of microRNAs across gestation	International Journal of Molecular Medicine (2020)	Ana Sofía Herrera-Van Oostdam, Juan Carlos Toro-Ortíz, et al.
Comparing small urinary extracellular vesicle purification methods with a view to RNA sequencing—Enabling robust and non-invasive biomarker research	Biomolecular Detection and Quantification (2019)	Veronika Mussack, Georg Wittmann, Michael W. Pfaffl