Thermo Fisher Scientific Beclin 1 Monoclonal Antibody (2A4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2A4

Immunogen

Purified recombinant fragment of human BECN1 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11153717

Product Specific Information

MA5-15825 targets BECN1 in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15825 immunogen is purified recombinant fragment of human BECN1 expressed in E. Coli.

MA5-15825 detects BECN1 which has a predicted molecular weight of approximately 60kDa.

Target Information

Beclin 1 plays a central role in autophagy. It acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Different PI3K complex forms are believed to play a role in multiple membrane trafficking pathways - PI3KC3-C1 is involved in the initation of autophagosomes and PI3KC3-C2 is involved in the maturation of autophagosomes and endocytosis. Beclin 1 also plays a role in antiviral host defense.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.