Thermo Fisher Scientific Beclin 1 Monoclonal Antibody (2A4)
Beclin 1 단백질을 표적하는 Thermo Fisher Scientific의 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry, ELISA 등에 사용 가능. 인간 시료에 반응하며 자가포식 연구에 적합. 장기 보관 시 -20°C에서 냉동 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1:200–1:400 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2A4 |
| Immunogen | Purified recombinant fragment of human BECN1 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_11153717 |
Product Specific Information
MA5-15825 targets BECN1 in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human BECN1 expressed in E. coli.
MA5-15825 detects BECN1, which has a predicted molecular weight of approximately 60 kDa.
Target Information
Beclin 1 plays a central role in autophagy as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate.
Different PI3K complex forms are involved in multiple membrane trafficking pathways:
- PI3KC3-C1 is involved in the initiation of autophagosomes.
- PI3KC3-C2 is involved in the maturation of autophagosomes and endocytosis.
Beclin 1 also contributes to antiviral host defense.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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