Thermo Fisher Scientific AP2M1 Recombinant Rabbit Monoclonal Antibody (ARC0522)
AP2M1 단백질을 인식하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체. Western blot, ICC/IF, ELISA 등 다양한 응용 가능. HEK293 세포에서 발현된 IgG 형식의 비결합 항체로 높은 특이성과 재현성을 제공. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| ELISA | 1 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0522 |
| Immunogen | Synthetic peptide corresponding to amino acids 300–400 of human AP2M1 (Q96CW1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.73 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848971 |
Product Specific Information
Immunogen sequence:
KLEVKVVIKS NFKPSLLAQK IEVRIPTPLN TSGVQVICMK GKAKYKASEN AIVWKIKRMA GMKESQISAE IELLPTNDKK KWARPPISMN FEVPFAPSGL K
Target Information
The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family.
The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping during endosome and lysosome acidification.
AP2M1 associates with the HIV-1 protein Nef, suggesting Nef may use the AP-2 complex to enhance endocytosis of CD4 and MHC class I molecules.
It also plays an important role in regulating intracellular trafficking and function of CTLA-4 protein.
At least two isoforms of AP2M1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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