Thermo Fisher Scientific alpha Tubulin Polyclonal Antibody
다양한 종(Human, Mouse, Rat, Zebrafish)에 반응하는 알파 튜불린 폴리클로날 항체. Western blot, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되어 높은 특이성 제공. 장기 보관 시 -20°C에서 안정적 유지.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 1:200–1:500 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within Human Alpha-tubulin aa 402–451 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 40% Glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2802723 |
Target Information
Alpha Tubulin forms a heterodimer with beta tubulin, each approximately 55 kDa in molecular weight. These heterodimers are the fundamental units of microtubules, which are essential cytoskeletal structures in eukaryotic cells. Microtubules provide structural support, facilitate intracellular transport, and play a crucial role in mitosis.
Gamma tubulin contributes to microtubule nucleation. Multiple alpha and beta tubulin genes are highly conserved across species. The alpha tubulin gene shares strong similarity with the mouse and rat Tuba1 gene, and expression is predominantly found in morphologically differentiated neuronal cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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