Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Biotin, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Biotin, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Brilliant UV series (395–805)
• Brilliant Violet series (421–786)
• eFluor series (450–615)
• FITC
• NovaFluor Blue & Yellow series
• Pacific Orange
• PE and PE derivatives (PE-Cyanine5, PE-Cyanine7, PE-eFluor 610 등)
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright series (436–780)
• Custom conjugation available upon request

Product Specific Information

Description:
The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by thymocytes, mature T cell subsets, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells, playing a key role in T cell development and activation. In T cells, CD4 associates with tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse thymocytes and splenocytes. Recommended ≤0.25 µg per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test, titration recommended for optimal performance.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD4 antigen is involved in recognition of MHC class II molecules and acts as a co-receptor for HIV. It is primarily expressed in T helper cells, but also found in monocytes, macrophages, and dendritic cells. CD4 expression is detectable in thymus, lymph nodes, tonsils, spleen, and certain non-lymphoid tissues. It assists in T-cell activation through association with the T-cell receptor complex and Lck kinase. CD4 may also mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.