Thermo Fisher Scientific Phospho-IRAK4 (Thr345) Monoclonal Antibody (A8A8)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

2-10 µg/mL

-

Product Specifications

Species Reactivity

Bovine, Dog, Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

A8A8

Immunogen

A synthetic peptide surrounding to the epitope -VMTSR- with a phosphorylation site at Thr345 of human IRAK-4 protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IRAK4,uniProtId:Q9NWZ3-1,ncbiNodeId:9606,antigenRange:343-347,antigenLength:460,antigenImageFileName:600-560_IRAK4_Q9NWZ3-1_House_mouse.svg,antigenImageFileNamePDP:600-560_IRAK4_Q9NWZ3-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

25mM tris with proprietary stabilizer

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Positive control: Jurkat Cell lysate

Cellular location: Cytoplasmic.

Target Information

IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. Overexpression of IRAK-4 activates NF-kappa-B and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.