
Thermo Fisher Scientific TEM7 Polyclonal Antibody
TEM7 단백질을 검출하기 위한 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제되었으며 PBS에 0.02% sodium azide 포함. 냉장 또는 냉동 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunohistochemistry (IHC) | 2.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 20 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 16 amino acid peptide from near the center of human TEM7 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long term storage store at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11157203 |
Product Specific Information
- A suggested positive control is rat liver tissue lysate.
- PA5-20428 can be used with blocking peptide PEP-0545.
Target Information
Using SAGE (Serial Analysis of Gene Expression) technology, St. Croix et al. identified 46 genes with elevated expression in tumor-associated endothelium. Nine of these genes were prominently expressed only in tumor endothelial cells (EC) and named Tumor Endothelial Markers (TEMs, TEM1–9).
TEM7 (Tumor endothelial marker 7) transcripts are specifically expressed in the endothelium of colorectal cancer, and primary cancers of lung, pancreas, breast, and brain. TEM7 expression is consistent across both primary and metastatic cancers. Other family members (TEM1, 3, 4, 5, 8, 9) show similar expression patterns in lung and brain tumors, and liver metastasis.
Since many of the genes differentially expressed in tumor endothelium are also expressed during angiogenesis, these genes may provide important resources for basic and clinical studies of human angiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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