Thermo Fisher Scientific HLA-DR Monoclonal Antibody (LN3), PE-Cyanine7, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.015 µg)/test
• Publications: View 25 publications

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Product Specifications

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Available Formats

• Unconjugated
• Alexa Fluor 660, 700, Plus 488, Plus 647
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 421–786
• eFluor 450, 506
• FITC
• NovaFluor Blue, UV, Violet, Yellow series
• PE, PE-Cyanine5, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436–780
• Request custom conjugation

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Product Specific Information

Description:
The LN3 monoclonal antibody reacts with human MHC class II molecule HLA-DR, expressed on antigen-presenting cells (B cells, monocytes, macrophages, dendritic cells, and activated T cells). HLA-DR is a heterodimeric transmembrane protein essential for peptide presentation to CD4⁺ T lymphocytes.

Applications Reported:
Validated for flow cytometric analysis.

Applications Tested:
Pre-titrated and tested using normal human peripheral blood cells. Use 5 µL (0.015 µg) per test (final volume 100 µL, 10⁵–10⁸ cells/test).

Light Sensitivity:
Protect from light; tandem dye is sensitive to photo-induced oxidation.

Fixation:
Samples may be stored in:

• IC Fixation Buffer (Product #00-8222-49)
• 1-step Fix/Lyse Solution (Product #00-5333-54)
  for up to 3 days at 4°C in the dark.

Excitation / Emission / Laser:
488–561 nm / 775 nm; Blue, Green, Yellow-Green lasers.

Filtration:
0.2 µm post-manufacturing filtered.

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Target Information

HLA-DR is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and a 27 kDa beta chain (DRB).

• Alpha chain (DRA): Non-polymorphic, acts as sole alpha chain for DRB1, DRB3, DRB4, DRB5.
• Beta chain (DRB): Contains polymorphisms determining peptide-binding specificity.
• Expression: Primarily on antigen-presenting cells (B lymphocytes, monocytes, macrophages, thymic epithelial cells, activated T lymphocytes).
• Function: Binds processed peptides, presents to T-helper cells, critical for immune response initiation.
• Applications: Widely used in immunopeptidomics for neoantigen identification via LC-MS/MS.

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For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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