Thermo Fisher Scientific CD278 (ICOS) Monoclonal Antibody (C398.4A), PE-Cyanine7, eBioscience

Applications

Tested Application

• Flow Cytometry (Flow)

Tested Dilution

• 0.5 µg/test
• Tested by flow cytometric analysis of stimulated mouse splenocytes.

View 6 publications

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Product Specifications

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Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• Super Bright 436
• Request custom conjugation

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Product Specific Information

The C398.4A monoclonal antibody reacts with ICOS (Inducible Costimulatory molecule), also known as H4/CRP-1/AILIM, and cross-reacts with mouse and rat ICOS.
ICOS is a T cell-specific activation molecule and a member of the CD28/CTLA-4 family. It is expressed on activated T cells, providing costimulatory activity for T cell activation and proliferation, and is essential for humoral immune responses.

C398.4A shows:

• Strong costimulation of proliferation in T cell clones
• Intermediate costimulation in activated T cells
• Weak costimulation in resting T cells
• High IL-10 production from pre-activated cells expressing high ICOS levels

The epitope recognized by C398.4A differs from that bound by ICOSL and does not block ICOSL binding.

Applications Reported: Flow cytometric analysis
Applications Tested: ≤0.5 µg/test (100 µL final volume; 10⁵–10⁸ cells/test recommended)

Light Sensitivity: Protect from light due to tandem dye photo-oxidation.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-8222-49)
• 1-step Fix/Lyse Solution (Product #00-5333-54)

Excitation / Emission: 488–561 nm / 775 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

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Target Information

ICOS (CD278) encodes a member of the CD28 and CTLA-4 receptor family. It functions as a homodimer and plays a key role in cell-cell signaling, immune response regulation, and cell proliferation.
Mutations or dysregulation of ICOS have been associated with immunodeficiency disorders such as Common Variable Immunodeficiency (CVID).

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For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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