Thermo Fisher Scientific Ki67 Monoclonal Antibody (OTI8G3), TrueMAB

Applications

• Immunohistochemistry (Paraffin) (IHC (P)): Tested dilution 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended product: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

Ki-67 is a nuclear protein expressed during various stages of the cell cycle—particularly in late G1, S, G2, and M phases.
It contains a forkhead-associated (FHA) domain that mediates association with euchromatin, the perichromosomal layer, centromeric heterochromatin, and the nucleolus.
Ki-67 shows a cell cycle-dependent localization pattern:

• Perinucleolar expression at G1
• Nuclear matrix expression at G2
• Chromosomal expression during M phase

As a proliferation marker, Ki-67 is absent in G0 cells and increases steadily from G1 through mitosis.
Ki-67 antibodies are widely used to determine the proliferative fraction in neoplasms and have prognostic relevance comparable to thymidine-labelling index.
Low Ki-67 index correlates strongly with histologically low-grade tumors.
It is also routinely applied as a neuronal marker for cell cycling and proliferation studies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.