Thermo Fisher Scientific TNNI3 Monoclonal Antibody (OTI4E5), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2500–1:5000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:50–1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended desalting product: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

Cardiac Troponin I (TNNI3) is a cardiac-specific inhibitory protein forming a complex with troponin C (TnC) and troponin T (TnT) in muscle thin filaments.
Calcium binding to TnC releases the inhibitory region of TnI from actin, enabling myosin attachment and muscle contraction.
Measurement of blood TnI and TnT levels serves as a diagnostic indicator of cardiac muscle damage, distinguishing angina from myocardial infarction.
Cardiac Troponin I also inhibits angiogenesis in vivo and in vitro.
Mutations in the TNNI3 gene are associated with familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.