Thermo Fisher Scientific CD28 Monoclonal Antibody (CD28.2), NovaFluor Yellow 755, eBioscience

Applications and Tested Dilution

Flow Cytometry (Flow)

• Tested Dilution: 5 µL (0.8 µg)/test

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Product Specifications

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Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 615 / 737
• Brilliant Violet 480 / 650 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S / 660-120S / 690
• NovaFluor Red 710 / 725 / 755
• NovaFluor Yellow 660
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 645
• Custom conjugation available

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Product Specific Information

The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells, and plasma cells. CD28 acts as a ligand for CD80 (B7-1) and CD86 (B7-2), serving as a potent co-stimulator of T cell activation. Signaling through CD28 enhances IL-2 and IL-2 receptor expression and increases cytotoxicity of CD3-activated T cells.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported

• Flow cytometric analysis

Applications Tested

• Pre-titrated for flow cytometric analysis of normal human peripheral blood cells
• Recommended use: 5 µL (0.8 µg) per test in 100 µL final volume
• Cell number range: 10⁵–10⁸ cells/test

Important Notes

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for compatibility.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for optimized fluorescence and minimal spectral spillover.

Excitation: 552 nm
Emission: 690 nm
Laser: 561 nm (Yellow Laser)

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Target Information

CD28 antigen is a 44 kDa disulfide-linked homodimeric T cell surface glycoprotein, belonging to the immunoglobulin superfamily. It is constitutively expressed on most peripheral T lymphocytes and serves as a key co-stimulatory receptor by binding CD80 and CD86 on antigen-presenting cells. CD28 signaling prevents T cell anergy and apoptosis, supporting immune activation.

High expression is found in thymocytes and immature T cells, with lower levels in mature CD4⁺ and CD8⁺ T cells. CD28 dysfunction is associated with diseases such as mycosis fungoides and Sezary’s disease.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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