Thermo Fisher Scientific OCT4 Recombinant Rabbit Monoclonal Antibody (3H8L6)
OCT4 단백질을 인식하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체. Western blot, ICC/IF, ChIP 등 다양한 응용에 적합. 높은 특이성과 일관성, 동물 유래 성분 없는 포뮬레이션. 줄기세포 연구 및 iPS 세포 생성 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL | View 3 publications |
| ChIP assay (ChIP) | 2.5 µg × 10⁶ cells | – |
| in situ PLA (PLA) | – | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Chicken, Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 3H8L6 |
| Immunogen | Protein corresponding to Human POU5F1 (aa 1–360) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2633031 |
Product Specific Information
This antibody is predicted to react with Monkey, Goat, Cat, and Horse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, and selected clones are screened for optimal performance.
Key advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broad immunoreactivity due to the rabbit immune repertoire
Target Information
POU5F1 (Oct-4) is a maternally expressed octamer-binding transcription factor essential in early embryonic development. It plays a critical role in maintaining pluripotency and self-renewal in stem cells.
Expression of POU5F1, along with Sox2, Klf4, Lin28, and c-Myc, enables the generation of induced pluripotent stem (iPS) cells from somatic cells. These iPS cells are valuable for regenerative medicine and cell replacement therapies, offering a virus-free, safer approach to stem cell generation.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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