Thermo Fisher Scientific Nkx3.1 Monoclonal Antibody (UMAB196), UltraMAB
Nkx3.1 단백질을 인식하는 Thermo Fisher Scientific의 UMAB196 단클론 항체. Western blot, IHC, ICC, ChIP 등 다양한 응용에 적합. 인간 시료 반응성, E.coli에서 발현된 full-length 단백질 면역원 사용. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution / Condition |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 |
| ChIP assay (ChIP) | Assay-dependent |
| Peptide Array (Array) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB196 |
| Immunogen | Full-length human recombinant protein of NKX3-1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
NKX3-1 is a homeodomain-containing transcription factor and a putative prostate tumor suppressor. It is expressed in a largely prostate-specific and androgen-regulated manner. Loss of NKX3-1 expression is commonly observed in human prostate carcinomas and prostatic intraepithelial neoplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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