Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Brilliant Violet 605, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Brilliant Violet 605, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780, Biotin
• Brilliant UV 395–805 Series
• Brilliant Violet 421–786 Series
• eFluor 450–660 Series
• FITC, Functional Grade
• NovaFluor Blue, Red, Violet, Yellow Series
• PE, PE-Cyanine Series, PerCP Series
• Super Bright Series
• Custom Conjugation Available

Product Specific Information

Description:
The 53-6.7 monoclonal antibody recognizes mouse CD8a, a 32–34 kDa cell surface receptor expressed as CD8αβ heterodimer or CD8αα homodimer. CD8 binds MHC class I and associates with tyrosine kinase p56lck, playing a key role in T cell development and activation.

Applications Reported:
Validated for flow cytometric analysis.

Applications Tested:
Tested on mouse splenocytes. Use ≤0.5 µg/test (100 µL volume). Cell count: 10⁵–10⁸ cells/test. Optimize titration for best results.

Fluorochrome Information:
Brilliant Violet™ 605 (BV605) emits at 605 nm, excitable by violet (405 nm) laser. Ensure instrument compatibility.

Buffer Recommendations:
For panels using multiple polymer dyes (Super Bright™, Brilliant Violet™, Brilliant UV™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Handling Notes:

• Light-sensitive: protect from photo-oxidation.
• Fixation: compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54).
• Store fixed samples ≤3 days at 4°C in dark.
• Not compatible with methanol-based fixation.

Laser Compatibility:

• Excitation: 407 nm
• Emission: 605 nm
• Laser: Violet

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein critical for T cell signal transduction, differentiation, and activation.

• Expressed mainly on T cells as CD8αβ heterodimer (MHC-I co-receptor).
• CD8α chain binds MHC-I.
• Also found on subsets of T cells, NK cells, monocytes, and dendritic cells as CD8αα homodimers.
• Interaction with MHC-I/peptide complexes activates Lck kinase, leading to CTL activation and pathogen/tumor clearance.
• Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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