Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Brilliant Violet 605, eBioscience
Mouse CD8a 단백질을 인식하는 53-6.7 클론의 Rat IgG2a 단일클론 항체로, Brilliant Violet 605 형광체로 결합되어 있습니다. Flow Cytometry 분석에 최적화되어 있으며, 405 nm 레이저에서 605 nm 방출을 제공합니다. 4°C 암소 보관, 메탄올 고정 불가.
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Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Brilliant Violet 605, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry
- Tested Dilution: 0.5 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 53-6.7 |
| Conjugate | Brilliant Violet™ 605 |
| Excitation / Emission Max | 406 / 603 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2937150 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780, Biotin
- Brilliant UV 395–805 Series
- Brilliant Violet 421–786 Series
- eFluor 450–660 Series
- FITC, Functional Grade
- NovaFluor Blue, Red, Violet, Yellow Series
- PE, PE-Cyanine Series, PerCP Series
- Super Bright Series
- Custom Conjugation Available
Product Specific Information
Description:
The 53-6.7 monoclonal antibody recognizes mouse CD8a, a 32–34 kDa cell surface receptor expressed as CD8αβ heterodimer or CD8αα homodimer. CD8 binds MHC class I and associates with tyrosine kinase p56lck, playing a key role in T cell development and activation.
Applications Reported:
Validated for flow cytometric analysis.
Applications Tested:
Tested on mouse splenocytes. Use ≤0.5 µg/test (100 µL volume). Cell count: 10⁵–10⁸ cells/test. Optimize titration for best results.
Fluorochrome Information:
Brilliant Violet™ 605 (BV605) emits at 605 nm, excitable by violet (405 nm) laser. Ensure instrument compatibility.
Buffer Recommendations:
For panels using multiple polymer dyes (Super Bright™, Brilliant Violet™, Brilliant UV™), use:
- Super Bright Complete Staining Buffer (Product # SB-4401-42)
- Brilliant Stain Buffer™ (Product # 00-4409-75)
Handling Notes:
- Light-sensitive: protect from photo-oxidation.
- Fixation: compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54).
- Store fixed samples ≤3 days at 4°C in dark.
- Not compatible with methanol-based fixation.
Laser Compatibility:
- Excitation: 407 nm
- Emission: 605 nm
- Laser: Violet
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein critical for T cell signal transduction, differentiation, and activation.
- Expressed mainly on T cells as CD8αβ heterodimer (MHC-I co-receptor).
- CD8α chain binds MHC-I.
- Also found on subsets of T cells, NK cells, monocytes, and dendritic cells as CD8αα homodimers.
- Interaction with MHC-I/peptide complexes activates Lck kinase, leading to CTL activation and pathogen/tumor clearance.
- Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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