Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Brilliant Violet 605, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 661, 737
• Brilliant Violet 480
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule (55 kDa), a cell surface receptor expressed on most thymocytes, subsets of mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and is essential for T cell development and function. Binding of GK1.5 is blocked by RM4-5.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤0.5 µg/test (100 µL final volume; 10⁵–10⁸ cells/test)

Brilliant Violet™ 605 (BV605) emits at 605 nm and is suitable for cytometers with a 405 nm violet laser. Protect from light due to photo-induced oxidation sensitivity.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Fixation: Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-54)

Brilliant Violet™ 605 is not compatible with methanol-based fixation.
Excitation: 407 nm Emission: 605 nm Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company. Powered by Sirigen™.

Target Information

CD4 is a co-receptor for MHC class II molecules and HIV, primarily expressed on T helper cells, monocytes, macrophages, and dendritic cells. It mediates T-cell activation through association with the T-cell receptor complex and kinase Lck. CD4 expression is found in thymus, lymph nodes, tonsils, spleen, and other tissues. Multiple alternatively spliced transcripts exist (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures.

제품 이미지