Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Brilliant Violet 605, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Brilliant Violet 605

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532, 561, 647, 660, 700
• APC, APC-eFluor 780, Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–786)
• eFluor series (450–615)
• FITC
• NovaFluor Blue/Yellow series
• Pacific Orange
• PE, PE-Cyanine series
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright series (436–780)
• Custom conjugation available

Product Specific Information

The RM4-5 monoclonal antibody reacts with mouse CD4, a 55 kDa surface receptor expressed on thymocytes, subsets of mature T cells, and dendritic cells. CD4 binds MHC class II on antigen-presenting cells and is critical for T cell development and function. RM4-5 binding is blocked by GK1.5.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes (≤0.25 µg/test, 100 µL final volume)
• Recommended: Titrate for optimal performance

Fluorophore Characteristics:
Brilliant Violet™ 605 (BV605) emits at 605 nm and is suitable for cytometers with a 405 nm violet laser. Protect from light due to photo-induced oxidation sensitivity.

Buffer Recommendations:
When using multiple polymer dyes (Super Bright, Brilliant Violet™, Brilliant UV™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to reduce non-specific interactions.

Fixation Guidelines:
Samples may be stored up to 3 days at 4°C in IC Fixation Buffer (Product # 00-8222-49) or Fix/Lyse Solution (Product # 00-5333-54). BV605 is not compatible with methanol-based fixation.

Optical Properties:

• Excitation: 407 nm
• Emission: 605 nm
• Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD4 antigen is a co-receptor for MHC class II and HIV, primarily expressed on T helper cells, monocytes, macrophages, and dendritic cells. It facilitates T-cell activation via association with the T-cell receptor complex and Lck kinase. CD4 is detected in thymus, lymph nodes, tonsils, spleen, and various non-lymphoid tissues. It may also contribute to neuronal damage in immune-mediated CNS diseases. Multiple alternatively spliced transcripts are known (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures.

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