Thermo Fisher Scientific ADORA1 Polyclonal Antibody
Thermo Fisher Scientific의 ADORA1 Polyclonal Antibody는 인간 및 마우스 시료에서 Adenosine Receptor A1 단백질을 검출합니다. Western blot 및 면역세포염색에 적합하며, 비결합(Unconjugated) 형태로 제공됩니다. -20°C에서 보관하며 반복 동결/해동은 피해야 합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:250 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptides corresponding to the C-terminal portion of human and mouse Adenosine Receptor A1 (Human: C(309)QPAPPIDEDLPEERPDD(326), Mouse: C(309)QPKPPIEEDIPEEKADD(326)) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Whole serum |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2222210 |
Product Specific Information
PA3-041 detects Adenosine Receptor A1 from human and mouse samples. Reactivity is stronger in mouse and is recommended for use in overexpressed or recombinant human samples.
It has been successfully used in Western blot applications, detecting a ~34–37 kDa band representing Adenosine Receptor A1 from mouse brain.
Target Information
Adenosine receptors (ARs) belong to the 7-transmembrane domain G-protein-coupled receptor superfamily. Four subtypes (A1, A2a, A2b, A3) have been identified, each mediating specific physiological processes such as cardiac rate regulation, smooth muscle tone, platelet aggregation, inflammation, cell growth, and neurotransmission.
The A1AR subtype activates Gi and Go proteins, leading to inhibition of adenylyl cyclase and stimulation of phospholipase C, resulting in intracellular calcium mobilization. It also activates potassium channels, influencing cardiovascular responses.
A1AR is highly expressed in brain (cerebellum, hippocampus, thalamus, cortex), spinal cord, kidney, testis, and white adipocytes, where it modulates neurotransmitter release, inhibits lipolysis, and stimulates glucose uptake.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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