Thermo Fisher Scientific SUPT6H Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunohistochemistry (IHC)

• Tested Dilution: 1:200–1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:200–1:1,000

Immunoprecipitation (IP)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Based on 100% sequence identity, this antibody is predicted to react with D. melanogaster and Zebrafish.

Target Information

Transcription elongation factor which binds histone H3 and plays a key role in the regulation of transcription elongation and mRNA processing. Enhances transcription elongation by RNA polymerase II (RNAPII) and is required for activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat.
Acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain (CTD) of RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) and histone modifying enzymes (SETD2) to ensure proper mRNA splicing, efficient mRNA export, and elongation-coupled H3K36 methylation.
SUPT6H regulates class-switch recombination and somatic hypermutation through formation of H3K4me3 marks on AICDA target loci via association with SETD1A. Also promotes activation of the myogenic gene program by erasing repressive H3K27me3 marks through stabilization of KDM6A interaction.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.