Thermo Fisher Scientific CD80 Monoclonal Antibody (RM80)

Applications

Immunohistochemistry (Frozen) (IHC (F))

• Assay-dependent

Flow Cytometry (Flow)

• Dilution: 1:10–1:50

Product Specifications

Product Specific Information

For FACS analysis, use 10 µL of the suggested working dilution to label 1×10^6 cells in 100 µL.

Rat anti-Mouse CD80 antibody, clone RM80 recognizes mouse CD80 (B7-1), an approximately 60 kDa cell surface glycoprotein which is a member of the CD28/B7 family.

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands of the T cell costimulatory molecule CD28 and the inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, being able to substitute for each other in this process.

CD80 is rapidly induced on the surface of in vitro activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes by binding to CD28 and CTLA4 ligands on T cells.

Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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