Thermo Fisher Scientific TLR7 Monoclonal Antibody (4F4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

Immunohistochemistry (IHC)

-

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Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

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Product Specifications

Species Reactivity

Human

Published species

Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4F4

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 10% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_935719

Product Specific Information

In Western blot, this antibody detects a band at ~121 kDa in Ramos cell lysate.

Mouse anti Human TLR7 antibody, clone 4F4 recognizes human TLR7 (toll-like receptor 7), a 121 kDa type I transmembrane protein belonging to the toll-like receptor (TLR) family.

Target Information

TLR7 is a 10 amino acid long highly conserved membrane protein belonging to the Toll-like receptor (TLR) family with 27 LRR (leucine-rich) repeats and a TIR domain. TLR7 plays a fundamental role in pathogen recognition and activation of innate immunity. TLR7 is activated by infections with single-stranded RNA viruses, including influenza virus and vesicular stomatitis virus (VSV). TLR7 is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8. TLR7 also acts via MyD88 and TRAF6, leading to NF-kappaB activation, cytokine secretion and the inflammatory response. TLR7 recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.