Thermo Fisher Scientific Phospho-Zap-70 (Tyr315) Polyclonal Antibody
ZAP-70 단백질의 Tyr315 인산화 부위를 특이적으로 인식하는 Rabbit Polyclonal Antibody입니다. Western blot 및 IHC(P)에서 사용 가능하며, 인간, 마우스, 랫트에 반응합니다. 항원 친화 크로마토그래피로 정제된 고순도 제품으로, 연구용으로 적합합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human ZAP70 around the phosphorylation site of Tyrosine 315 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553799 |
Product Specific Information
This antibody detects endogenous protein at a molecular weight of approximately 70 kDa.
Purity is >95% by SDS-PAGE.
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation.
Upon TCR engagement, ZAP70 is phosphorylated on tyrosines 292, 315, and 319 in the interdomain B region between SH2 and kinase domains.
Phosphorylation of tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances signaling, while phosphorylation at tyrosine 292 attenuates activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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