Thermo Fisher Scientific VPS24 Polyclonal Antibody
Rabbit polyclonal antibody against human VPS24 (CHMP3) for WB and IHC applications. Reacts with human, mouse, and rat. Lyophilized form, unconjugated, purified by ammonium sulfate precipitation. Suitable for research use only.
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Thermo Fisher Scientific VPS24 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 10–50 µg/mL |
| Immunohistochemistry (IHC) | 10–50 µg/mL |
| Miscellaneous (PubMed) | – |
Product Specifications
| Category | Details |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from the N-terminal of human VPS24 conjugated to an immunogenic carrier protein. Target family: CHMP3, UniProt ID: Q9Y3E7-1, antigen range: 1–222. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
- The antigen is homologous in rat and mouse.
- Specificity: CHMP3.
Target Information
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III), involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs.
MVBs contain intraluminal vesicles (ILVs) generated by invagination and scission from the limiting membrane of the endosome, delivered to lysosomes for degradation of membrane proteins, lysosomal enzymes, and lipids.
The MVB pathway requires sequential function of ESCRT-O, -I, -II, and -III complexes.
ESCRT-III proteins dissociate before ILV release and also function in membrane fission events such as cytokinesis and viral budding (e.g., HIV-1).
ESCRT-III proteins mediate vesicle extrusion and membrane fission, possibly with AAA ATPase VPS4.
Selectively binds phosphatidylinositol 3,5-bisphosphate [PtdIns(3,5)P2] and PtdIns(3,4)P2.
Involved in late stages of cytokinesis and endosomal sorting/trafficking of EGF receptor.
Isoform 2 prevents stress-mediated cell death and accumulation of reactive oxygen species in yeast cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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