Thermo Fisher Scientific PPARA Monoclonal Antibody (OTI1E8), TrueMAB
PPARA 단백질을 인식하는 Mouse monoclonal antibody로 Western Blot 및 Immunocytochemistry에 적합. Human과 Mouse 시료에 반응하며, Lyophilized 형태로 공급. 고순도 친화 크로마토그래피 정제 및 안정적 저장 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:200–1:500
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 145–420 of human PPARA produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL distilled water to obtain a final concentration of approximately 1 mg/mL.
- For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Peroxisome proliferators are non-genotoxic carcinogens that act through nuclear hormone receptors known as peroxisome proliferator-activated receptors (PPARs). These ligand-dependent intracellular proteins regulate gene transcription by binding to specific DNA sequences upon activation. PPARs are activated by compounds such as clofibric acid, nafenopin, WY-14,643, and certain fatty acids. They can induce transcription of acyl-CoA oxidase and cytochrome P450 (CYP450) A6 through specific response elements. PPARs also form heterodimers with retinoid X receptor (RXR) alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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