Thermo Fisher Scientific Phospho-Paxillin (Ser126) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

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Immunohistochemistry (Paraffin) (IHC (P))

1:20

-

Immunocytochemistry (ICC/IF)

1:250

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Immunoprecipitation (IP)

-

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Product Specifications

Species Reactivity

Chicken, Human, Mouse

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human paxillin that contains serine 126. The sequence is conserved in mouse and chicken. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Paxillin,uniProtId:P49024-1,ncbiNodeId:9031,antigenRange:126,antigenLength:559,antigenImageFileName:44-1022G_Paxillin_P49024-1_Rabbit.svg,antigenImageFileNamePDP:44-1022G_Paxillin_P49024-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco`s PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533553

Target Information

Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.