Thermo Fisher Scientific Phospho-Histone H1.4 (Ser35) Polyclonal Antibody
Phospho-Histone H1.4 (Ser35)에 특이적인 폴리클로날 항체로, 인간 시료에 반응. Western blot, IHC, ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제된 고순도 항체. 연구용으로 세포 내 히스톤 인산화 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human Histone H1S35ph (phospho Ser35) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.93 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2549380 |
Product Specific Information
- Recommended positive control: Acid-extracted histones from HeLa cells treated with Nocodazole
- Store product as a concentrated solution. Centrifuge briefly before opening the vial.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes.
The linker histone H1 interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher-order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts lack polyA tails but contain a palindromic termination element. This gene is located in the large histone gene cluster on chromosome 6.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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