Thermo Fisher Scientific POLI Monoclonal Antibody (OTI5A4), TrueMAB
Human POLI 단백질(aa 458-740)에 특이적인 Mouse monoclonal antibody. Western blot에 1:2,000 희석으로 사용 가능. Lyophilized 형태, PBS buffer(8% trehalose) 포함. DNA 손상 복구 연구용으로 적합.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5A4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 458–740 of human POLI produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis when high-fidelity DNA polymerases stall. Favors Hoogsteen base-pairing in the active site and inserts the correct base opposite an adenosine template with high fidelity. Exhibits low fidelity opposite a thymidine template, preferentially inserting guanosine. May contribute to immunoglobulin gene hypermutation. Forms a Schiff base with 5′-deoxyribose phosphate at abasic sites but may lack lyase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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