Thermo Fisher Scientific POLI Monoclonal Antibody (OTI3B5), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis when normal high-fidelity DNA polymerases stall.
Favors Hoogsteen base-pairing in the active site and inserts the correct base opposite adenosine templates with high fidelity.
Shows low fidelity opposite thymidine templates, preferentially inserting guanosine.
May be involved in hypermutation of immunoglobulin genes and can form a Schiff base with 5'-deoxyribose phosphate at abasic sites, though it may lack lyase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.