Thermo Fisher Scientific AMPK alpha 1 Polyclonal Antibody
Rabbit polyclonal antibody targeting AMPK alpha 1 for WB, IHC, and IP applications. Recommended IHC dilution 1:100–1:500 with citrate buffer retrieval. Liquid form, 0.10 mg/mL concentration, stored at 4°C. Predicted cross-reactivity with orangutan.
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- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Between amino acids 350 and 400 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.10 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Predicted to react with orangutan based on 100% sequence identity
Target Information
Protein kinases are enzymes that transfer a phosphate group from a donor, typically the γ-phosphate of ATP, to an acceptor amino acid in a substrate protein. This phosphorylation mechanism mediates signal transduction in eukaryotic cells, influencing metabolism, transcription, cell cycle, cytoskeletal rearrangement, cell movement, apoptosis, and differentiation.
The protein kinase family includes over 500 gene products and is one of the largest in eukaryotes, classified into eight major groups based on sequence similarity of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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