Thermo Fisher Scientific Phospho-FAK (Ser732) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling.
FAK plays an important role in:

• Cell spreading and differentiation
• Migration and cell death
• G1 to S phase transition of the cell cycle

FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, sites where cells attach to the extracellular matrix via surface integrin receptors.
Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, resulting in activation of the ERK2 MAP kinase.
In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin.
In vitro, the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, with Tyr925 identified as the binding site by site-directed mutagenesis.
Several transcript variants encoding different isoforms have been found for the FAK gene, with three full-length forms determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.