Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), eFluor 570, eBioscience
마우스 IgM의 μ 중쇄를 인식하는 단클론 항체로, 다른 면역글로불린과 반응하지 않음. IHC, ICC, ELISA 등 다양한 응용에 적합. eFluor 570 형광 표지로 고감도 검출 가능. 4°C 암소 보관, 동결 금지. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 4 publications |
| Immunohistochemistry (Frozen) (IHC-F) | 10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | 2 publications |
| ELISA | - | 5 publications |
| T-Cell Activation (TCA) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 570, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | II/41 |
| Conjugate | eFluor™ 570 |
| Excitation / Emission Max | 556 / 569 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573616 |
Additional Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 605
- Brilliant Violet 711
- eFluor 450
- eFluor 660
- FITC
- NovaFluor Red 710
- NovaFluor Yellow 610
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 780
- Request custom conjugation
Product Specific Information
The II/41 monoclonal antibody reacts with the μ heavy chain of mouse IgM and does not cross-react with other mouse immunoglobulin classes (IgD, IgG, IgA). IgM is expressed intracellularly in early B cell development and on mature B cells in bone marrow and peripheral tissues.
Applications Reported:
- Immunohistochemical staining of frozen tissue sections
- Immunocytochemistry
Applications Tested:
- Tested by ICC on fixed, differentiated OLN93 cells with O1 (Anti-Oligodendrocyte Marker) primary antibody
- Tested by IHC-P on FFPE human tonsil with HI98 (Anti-CD15) primary antibody
- Recommended concentration ≤ 10 µg/mL (optimize per assay)
Filter Recommendation:
For eFluor™ 570 detection, use filter capturing 570 nm emission (e.g., Excitation 545/25, 565LP, Emission 605/70).
Standard Alexa Fluor™ 555 or TRITC filters are also suitable.
Excitation: 555 nm
Emission: 570 nm
Target Information
Mouse IgM is an immunoglobulin M antibody produced by B cells in response to antigens. It is the first antibody generated during immune response and the largest immunoglobulin in serum. Composed of five heavy and five light chains, IgM plays a key role in complement activation and antigen agglutination. It is essential for innate immune defense and is widely used in ELISA, Western blotting, and immunohistochemistry research applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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