Thermo Fisher Scientific CD34 Monoclonal Antibody (4H11), PE-Cyanine7, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test
• Publications: View 58 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• PE
• PE-Cyanine5
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The 4H11 monoclonal antibody reacts with human CD34, also known as mucosialin. CD34 belongs to a protein family including endoglycan and podocalyxin, characterized by single-pass transmembrane structure and glycosylated extracellular domains. CD34 is a well-established marker for hematopoietic progenitors and is also expressed on certain mesenchymal stem cells, tumor cell lines, and vascular endothelia.

Epitopes of CD34 are categorized into three classes (I–III) based on enzymatic sensitivity; 4H11 belongs to class III, recognizing a protein epitope.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated for flow cytometry using normal human peripheral blood cells at 5 µL (0.25 µg)/test (in 100 µL final volume).

Light Sensitivity: Protect from light due to photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.

Excitation: 488–561 nm
Emission: 775 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD34 is a highly glycosylated monomeric surface protein (111–115 kDa) expressed on various stem cell populations. It serves as a marker for hematopoietic stem cells and may function as a receptor involved in adhesion, differentiation, and proliferation. CD34 possibly mediates stem cell attachment to bone marrow extracellular matrix or stromal cells and may act as a scaffold for glycans binding to lectins. The intracellular domain is a phosphorylation site for protein kinase C, suggesting a role in signal transduction.

Associated Diseases: Dermatofibrosarcoma, neurofibroma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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