Thermo Fisher Scientific CD38 Monoclonal Antibody (HIT2), PE-Cyanine7, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 5 µL (1 µg)/test
• View 39 publications

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• eFluor 450
• FITC
• PE
• PE-Cyanine5
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 702
• Request custom conjugation

Product Specific Information

The HIT2 monoclonal antibody reacts with the human CD38 molecule (~45 kDa type II transmembrane protein).
CD38 is expressed by thymocytes, peripheral B cells (including plasma cells), activated T cells, and monocytes. It acts as a counter-receptor for CD31 with both cyclase and hydrolase enzymatic activity.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
  • Recommended use: 5 µL (1 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
• Light Sensitivity: Protect from light (photo-induced oxidation).
• Fixation:
  • Use IC Fixation Buffer (Product # 00-8222-49) or
    1-step Fix/Lyse Solution (Product # 00-5333-54)
  • Store up to 3 days at 4°C in the dark.
• Excitation: 488–561 nm
• Emission: 775 nm
• Laser Compatibility: Blue, Green, Yellow-Green lasers
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD38 (NAD⁺ glycohydrolase) is a type II transmembrane glycoprotein that induces activation, proliferation, and differentiation of mature lymphocytes and mediates apoptosis of myeloid and lymphoid progenitor cells.
It functions as a multi-catalytic ectoenzyme (ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase, NAD⁺ glycohydrolase) and as a cell surface receptor.

Applications:

• Subtyping of lymphomas and leukemias
• Detection of plasma cells (e.g., myelomas)
• Marker for activated B and T cells
• Involved in cell adhesion, signal transduction, and calcium signaling

CD38 is highly expressed in pancreas, liver, kidney, malignant lymphoma, and neuroblastoma.
Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter’s Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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