Thermo Fisher Scientific IL-10 Monoclonal Antibody, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 1.0–2.0 µg/mL

ELISA

• Tested Dilution: 4.0–8.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGQKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLEDQGVYKA MNEFDIFINC IEAYMMIKMK S

Preparation:
Produced in Sprague Dawley rats immunized with highly pure Recombinant Murine IL-10. Anti-Murine IL-10-specific antibody was purified from cell culture by Protein G affinity chromatography.

Target Information

IL-10 encodes a cytokine primarily produced by monocytes and lymphocytes. It plays a key role in immunoregulation and inflammation by:

• Down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages
• Enhancing B cell survival, proliferation, and antibody production
• Blocking NF-kappa B activity
• Regulating the JAK-STAT signaling pathway

Knockout studies in mice indicate IL-10 is essential for intestinal immune regulation. Mutations in IL10 are associated with increased susceptibility to HIV-1 infection and rheumatoid arthritis. Related diseases include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.