Thermo Fisher Scientific NEK2 Polyclonal Antibody
NEK2 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, Western Blot, ICC/IF, ELISA, IP 등에 사용 가능. 인간 NEK2에 특이적이며, 액상 형태로 -20°C 보관. 세포주기 조절 및 유사분열 연구에 적합.
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Thermo Fisher Scientific NEK2 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to internal region near aa 275–300 of Human NEK2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
BLAST analysis indicates 100% homology of the immunizing sequence with NEK2 homologues from human and chimpanzee.
Cross-reactivity with NEK2 protein homologues from other sources may not occur as sequence homology varies by at least one amino acid residue.
Target Information
NEK2 is a serine/threonine-protein kinase involved in cell cycle regulation and essential for faithful chromosome segregation.
This protein is primarily located in the centrosome and plays a crucial role in mitosis regulation.
Its expression increases progressively during the S phase and peaks in late G2 phase.
The gene has several alternatively spliced transcript variants encoding distinct isoforms.
NEK2 is associated with diseases such as Retinitis Pigmentosa 67.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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