Thermo Fisher Scientific NEK2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500- 1:2,000

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

ELISA (ELISA)

1:2,000-1:10,000

-

Immunoprecipitation (IP)

1:500-1:2,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 275-300 of Human NEK2 protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NEK2,uniProtId:P51955-1,ncbiNodeId:9606,antigenRange:275-300,antigenLength:445,antigenImageFileName:600-401-452_NEK2_P51955-1_Rabbit.svg,antigenImageFileNamePDP:600-401-452_NEK2_P51955-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

BLAST analysis indicates 100 % homology of the immunizing sequence with NEK2 homologues from human and chimpanzee. Cross reactivity with NEK2 protein homologues from other sources may not occur as sequence homology varies by at least one amino acid residue in this sequence.

Target Information

NEK2 is involved in cell cycle regulation and is essential for faithful chromosome segregation. NEK2 is a gene that encodes for a serine/threonine-protein kinase. This protein plays a crucial role in the regulation of mitosis and is primarily located in the centrosome. During the G1 phase, the protein is undetectable, but its levels progressively increase throughout the S phase, reaching their peak in late G2 phase. The gene has several alternatively spliced transcript variants that encode different isoforms with distinct C-termini. NEK2 is associated with several diseases, including Retinitis Pigmentosa 67 and Retinitis Pigmentosa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.