Thermo Fisher Scientific Ldb2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500- 1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

-

ELISA (ELISA)

1:5,000-1:20,000

-

Immunoprecipitation (IP)

1:500-1:2,000

-

Product Specifications

Species Reactivity

Dog, Chicken, Chimpanzee, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 100-125 of Mouse LDB2. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LDB2,uniProtId:O55203-1,ncbiNodeId:10090,antigenRange:100-125,antigenLength:373,antigenImageFileName:600-401-459_LDB2_O55203-1_Rabbit.svg,antigenImageFileNamePDP:600-401-459_LDB2_O55203-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.06 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against mouse LDB2. A BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, rat and chicken based on 100% homology for the immunogen sequence. Cross reactivity with LDB2 homologues from other sources has not been determined.

Target Information

Genes encoding LIM domain-binding factors were initially isolated in a screen for proteins that physically interact with the LIM domains of nuclear proteins. These proteins, such as the one encoded by the LDB2 gene, are capable of binding to a variety of transcription factors and are likely to function at enhancers to bring together diverse transcription factors and form higher order activation complexes or to block formation of such complexes. The family of genes encoding LIM domain-binding factors includes 2 members isolated from the mouse, Clim1 and Clim2/Lbd1/Nli; and their homologs cloned from the frog, chicken, and fly. The fact that LIM domain-binding factors are likely to be involved in the coordination of the transcriptional activity of many diverse factors might implicate them in human phenotypes characterized by multiple affected sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.